Objective To investigate the species distribution of hematophagous midges in 31 counties (banners and cities) in 8 provinces (autonomous regions) of China, including Jiangxi and Yunnan. Methods From 2013 to 2018, hematophagous midges were captured with light traps at the residences, cattle pens, sheepfolds, chicken houses, pig pens, duck pens, and vegetable plots in 31 counties (banners and cities) in 8 provinces (autonomous regions) of China, including Jiangxi and Yunnan; specimens were slide-mounted in phenol-balsam. Results A total of 184 314 hematophagous midges from 72 species belonging to 2 genera were collected from 31 counties (banners and cities) in 8 provinces (autonomous regions) of China, of which 63 species belonged to the genus Culicoides and 9 species belonged to the genus Lasiohelea. There were 7 species in 3 counties (banners) in Inner Mongolia, 4 species in a county in Xinjiang, 16 species in a county in Guizhou, 9 species in 2 counties (cities) in Sichuan, 27 species in 10 counties (cities) in Jiangxi, 20 species in 3 counties in Hunan, 36 species in 6 counties (cities) in Yunnan, and 28 species in 5 counties (cities) in Hainan. In terms of distribution in provinces and cities, dominant midges species were distributed as follows: C. punctatus in 19 counties (cities) in 7 provinces (autonomous regions), C. homotomus in 18 counties (cities) in 7 provinces (autonomous regions), C. arakawai in 27 counties (cities) in 6 provinces (autonomous regions), C. oxystoma in 23 counties (cities) in 6 provinces (autonomous regions), C. parahumeralis in 12 counties (cities) in 6 provinces, and C. nipponensis in 12 counties (cities) in 5 provinces (autonomous regions). C. punctatus, C. homotomus, C. arakawai, C. oxystoma, and C. nipponensis each were distributed in cattle pens, sheepfolds, pig pens, duck pens, and residences. Conclusion Hematophagous midges from 72 species belonging to 2 genera are collected in 31 counties (banners and cities) in 8 provinces (autonomous regions) of China; C. punctatus, C. homotomus, C. arakawai, C. oxystoma, C. nipponensis, and C. parahumeralis are widely distributed hematophagous midge species in the 31 counties (cities) in 8 provinces (autonomous regions) under this investigation in China.

Objective To investigate the species distribution, species composition, blood-sucking rate, and light-trap index in the cattle pens in three northeastern provinces of China. Methods From 2010 to 2019, hematophagous midges were captured with light traps from cattle pens in 7 counties (cities) in three northeastern provinces of China. The Chi-square test was used to compare the blood-sucking rates. Results A total of 322 505 hematophagous midges were collected from 7 counties (cities) in northeast China, which belonged to 29 species from 2 genera, including 28 species of Culicoides and 1 species of Lasiohelea. Among them, Kuandian county had 19 species, Dongning city had 15 species, Xunke county had 15 species, Dunhua city had 8 species, Fuyuan county had 8 species, Qingyuan county had 6 species, and Ji'an city had 7 species. The dominant species were C. sinanoensis (38.42%) and C. punctatus (37.60%), and the species composition varied in different counties (cities). The overall blood-sucking rate of 9 main species in 4 counties (cities) was 54.63%. Specifically, C. orientalis had the highest blood-sucking rate (71.87%), and C. humeralis had the lowest (14.68%). The overall light-trap index in 5 counties (cities) was 4 788.98 midges/light·night. Specifically, Dunhua city had the highest light-trap index (9 499.94 midges/light·night), and Xunke county had the lowest (1 408.62 midges/light·night). The blood-sucking rate and light-trap index varied across different midge species and counties (cities). Conclusion There were 29 species from 2 genera of hematophagous midges in the cattle pens of 7 counties (cities) in three northeastern provinces of China. The dominant species were C. sinanoensis and C. punctatus. C. orientalis had the highest blood-sucking rate, and Dunhua city had the highest light-trap index.

Objective To investigate rodent population distribution and rodent control effectiveness in a working area of Japanese abandoned chemical weapons in eastern Jilin province, China. Methods The trapping method was used to capture rodents from June to August of 2016 in 3 habitats (mixed coniferous-broadleaf forests, marginal areas of cultivated land, and temporary settlements). The chi-square test was used to compare the capture rate of rodents in different habitats. Rodent control was conducted with poison baits in 4 zones of the working area and the control effectiveness was determined in July 2017. Results A total of 398 rodents were captured, involving 10 species from 8 genera. The dominant species were Apodemus agrarius (37.19%) and Tscherskia triton (32.66%), followed by Rattus norvegicus (9.30%) and A. peninsulae(8.54%). After 10 days of deratization, deratization rates in the 4 zones were 71.43%, 84.85%, 81.25%, and 91.30%, respectively, averaging 84.79%.The difference of mousetrap rate between different habitats and month was statistically significant ( χ ²=119.431, P<0.001; χ ²=15.872, P<0.001). Conclusion There were many kinds of rodents in this area, the density of rodents was high, and the effect of poison bait is remarkable.

This paper reports 22 species of hematophagous midges belonging to 2 genera collected by light trapping and human trapping in Lancang county, Yunnan province, China in September 2018, among which there were 18 species of Culicoides and 4 species of Lasiohelea. Culicoides kinari Howarth, 1985 was a new record in China. Identifying characteristics of C. kinari were as follows:wing length 1.15 mm, 11 pale spots and a radial cell on wings, antenna sensilla coeloconica present on segments 3 and 11-15, antennal ratio 1.09, without sensory pit in the third segment of the palpus, palpal ratio 4.29, and two oval spermathecae of the same size. C. shortti was recorded for the first time in Yunnan province. Specimens are deposited in the Center for Disease Control and Prevention of Northern Theater Command (Shenyang 110034, China).

Objective To investigate the species distribution and ecological habit of hematophagous midges in 10 counties or cities in the northeastern and midwestern Jiangxi province, China. Methods Hematophagous midges were captured in 10 counties or cities of Jiangxi (Lichuan, Zixi, Yingtan, Qianshan, Yushan, and Fuliang in the northeast as well as Yongfeng, Xiajiang, Anfu, and Jinggangshan in the midwest) using the light-trap method. The intact individuals of hematophagous midges were selected, and the specimens were slide-mounted in Canada balsam-phenol and subjected to taxonomic identification. Results A total 45 999 hematophagous midges belonging to 27 species in 2 genera were captured in the 10 counties or cities of Jiangxi from June to July, 2018, including 25 species in Culicoides and 2 species in Lasiohelea. Of the 27 species, 11 were recorded for the first time in Jiangxi: C. clavipalpis, C. flaviscutatus, C. innoxius, C. jacobsoni, C. luteolus, C. marginus, C. newsteadi, C. parahumeralis, C. tainanus, C. tenuipalpis, and Lasiohelea diaoluoensis. There were 26 species in the northeastern region and 13 species in the midwestern region. The dominant species were C. arakawai (45.31%) and C. oxystoma (16.71%). The dominant species in the northeastern region were C. arakawai (28.54%) and C. oxystoma (22.05%), and the dominant species in the midwestern region was C. arakawai (91.91%). The species distribution and dominant species varied in different places. The overall blood-sucking rate of 7 dominant species was 16.56%, of which, C. oxystoma had the highest blood-sucking rate (43.90%) and C. arakawai had the lowest blood-sucking rate (6.06%). The total light-trap index of hematophagous midges was 943.54/lamp in six sites, with the highest index in the cattle pen (1 297.78/lamp) and the lowest index in sheepfold (684.25/lamp). The blood-sucking rate and light-trap index varied with different midges species and places. Conclusion The faunal distribution and ecological habit of hematophagous midges in Jiangxi province have been mastered, which provides a reference for investigation as well as prevention and control of midge-borne diseases.

Objective To investigate the species composition and seasonality of blood-sucking midges in Bohai New Area of Cangzhou, Hebei province, China. Methods Blood-sucking midges were collected in Bohai New Area using light traps and insect nets from April to October, 2018, with slaughterhouses, quarantine farms, henhouses, hog houses, stables and residential areas as the selected observation points. Results A total of 5 107 blood-sucking midges, belonging to 1 genus and 7 species, were collected in Bohai New Area, of which Culicoides humeralis was recorded for the first time in Hebei province, and the dominant species was C. homotomus (72.22%). The species composition of blood-sucking midges varied between different places and months. The active season of blood-sucking midges was from the second half of April to the first half of October, and there were two peaks:the first half of June (53.72 inds/lamp) and the second half of August (71.88 inds/lamp). There were two peaks of activity, the first half of June (37.24 inds/lamp) and the second half of August (68.41 inds/lamp), for C. homotomus. There were two peaks of activity, the second half of May (16.08 inds/lamp) and the first half of August (23.22 inds/lamp), for C. morisitai. The total index of blood-sucking midges captured by the light traps from April to October was 21.46 inds/lamp, and the index of blood-sucking midges captured by the light traps varied across different months. Conclusion This investigation provides a reference for studies of the species composition and seasonal variation of blood-sucking midges and midge-borne diseases in the Bohai New Area of Cangzhou, Hebei province, China.

This article reports 23 species of hematophagous midges belonging to 2 genera in Wenchang city, Danzhou city, and Chengmai county of Hainan province, China in 2017, among which there were 22 species of Culicoides and 1 species of Lasiohelea. A new species of C. danzhouensis Liu et Liang, sp. nov. was found, and C. pangkorensis Wirth et Hubert, 1989 was a new record in China. The species of C. calcaratus Wirth et Hubert, 1989 was recorded for the first time in Hainan province. There were 17 species in Wenchang, 18 species in Danzhou, and 19 species in Chengmai. C. danzhouensis Liu et Liang sp. nov. had similar wings as C. recurvus Delfinado, 1961, but the latter had shorter palpus, with a palpus ratio of 2.2; male C. recurvus had narrow parameres, no horn process outside the near middle area, a papillae minute at the middle area of the rear edge of the ninth tergum, and no microtrichia on the ventral membrane of the ninth sternum, which were significantly different from C. danzhouensis Liu et Liang sp. nov. C. danzhouensis Liu et Liang sp. nov. had similar male terminalia as C. lushuiensis Liu et Feng, 2018, but there were significant differences in the numbers and shapes of pale spots on wings between the two species; the latter had no microtrichia on the ventral membrane of the ninth sternum, with aedeagus basal arch extending to nearly one-fifth of total length, which were significantly different from C. danzhouensis Liu et Liang sp. nov. The model specimen of the new species was deposited in the North Command Center for Disease Control and Prevention (Shenyang 110034)

This paper reports Culicoides ( Oecacta) fengxiangensis Liang, Liu et Zhang sp. nov. from Luobei county, Heilongjiang province, China. The new species is similar to C.( O.) subfascipennis Kieffer, 1919. However, the latter has the following characteristics:the pale spot in the middle of M2 cell is connected with the pale spot in the r-m, and the pale spot in the basal portion of anal cell is connected with the pale spot in the wing base; the rear edge of the ninth sternum in the male terminalia is arc-shaped, there are microchaeta on the membrane, the rear edge of the ninth tergum is widely dented, and the aedeagus is nearly arc-shaped. The model specimens of the new species are kept in the North Command Center for Disease Control and Prevention (Shenyang 110034).

This paper reports 24 species of the genus Culicoides of hematophagous midges in Heihe, Heilongjiang province, China. A new species C. aihuiensis Wu, Jiao et Liu, sp. nov., was found. The new species is similar to C. heiheensis Li, Zhang et Liu, 2011. However, the male C. heiheensis has a pair of blunt bulge at the rear edge of the ninth tergum, a wide arch-shaped aedeagus, a petal-shaped middle part, connected parameres, and a pair of cornicults in the middle part, which are substantially different from the new species. The model specimen of the new species has been deposited in the Center for Disease Control and Prevention of Shenyang Command (Shenyang 110034, China).

This paper reports 2 genera, 16 species of hematophagous midges from Lushui, Fugong, Gongshan, 3 counties of Yunnan province in China-Burma border, 2017, among which 15 species in Culicoides and 1 species in Lasiohelea. A new species C. ( Culicoides) lushuiensis Liu et Feng, sp.nov. is described. Three species: C. cylindratus, C. newsteadi, La. hainana are recorded for the first time in Yunnan province. Culicoides ( Culicoides) lushuiensis Liu et Feng, sp.nov. is closely allied to C. dubius Arnaud, 1956, but is distinctly different from distal portion anal cell of wing with a ouvm shap pale spot, PR 3.00 of female; and ninth sternum with wide "V" caudomedion excavationninth, tergum apically with fine break out, Basistyle with rectangulate ventra root, aedeagus distal portion short, stout, aedeagus basal arch extending nearly to half of total length of male of C. dubius Arnaud, 1956. The type specimens are deposited in the Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

This paper reports 2 genera, 20 species of hematophagous midges in 2017 in Hekou county, Yunnan province, China, among which 19 species in Culicoides and 1 species in Lasiohelea. A new record in China, C. luteolus Wirth et Hubert, 1989 is described. Five species, C. calvipalpis, C. innoxius, C. lingshuiensis, C. rugulithecus, and La. dirus are recorded for the first time in Yunnan province. Specimens are deposited in the Shenyang Command Center for Disease Control and Prevention(Shenyang 110034).

The paper reports 9 species of Culicoides ( Sinocoides)in China, a new species C. (S.)jinghongensis Wu et Liu, sp. nov. was described. The new species is closely allied to C. ( S.) multifarious Liu, Gong et Zhang, 2003, but it is distinctly different from the numbers and shape of pale spots in cell R5 and anal cell of wing female C. ( S.) jinghongensis Wu et Liu. The distribution of known species in China and mean values of certain numerical characters were recorded. The key list and shape figures to females of C. ( Sinocoides)were given. The type specimens were deposited in the Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

A new species and a new record of hematophagous midges, Culicoides enpingensis Wu et Liu, sp. nov., and C. calcaratus Wirth et Hubert, 1989 (new record in China)were described from Enping city of Guangdong province in China. Culicoides enpingensis Wu et Liu, sp. nov. is similar to C. lunchiensis Chen et Tsai, 1962, but distinctly different in the shapes of pale spots in cell R5, and base portion anal cell of wing; distal portion of aedeagus with a pair ear salient, and obtuse round at tip; parameres with hairy tip of male C. lunchiensis Chen et Tsai, 1962. The type specimens were deposited in the Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

A new species of Culicoides( Beltranmyia), C.( B.) shenyangensis sp. nov. is described from Shenyang city, Liaoning province in China. This new species is similar to C. pallidulus Yu, 1991, but distinctly different in the eyes with interfacetal hairs, wing with 7 pale spots, without lateral spinae in distal portion of aedeagus, parameres with bases separated of C. pallidulus Yu, 1991. Wing with pale sport of this new species is also somewhat similar to C. sibiricus Mirzaeva, 1964, but is distinctly different in the antenna sensilla coeloconica present on segments 3-15, AR 1.7, and shape of aedeagus and parameres of C. sibiricus Mirzaeva, 1964. The type specimens are deposited in the Center for Disease Control and Prevention Shenyang Command(Shenyang 110034).

This paper reports 2 genera, 16 species of hematophagous midges in 2016 in Dejiang county, Guizhou province, China, among which 13 species in Culicoides and 3 species in Lasiohelea. A new species C. ( Oecacta) qinglongensis Liu, sp. nov. was described. Five species, C. actoni, C. kongmiaoensis, C. newsteadi, C. parahumeralis, and La. danxinensis were recorded for the first time in Guizhou province. Culicoides ( O.) qinglongensis Liu, sp. nov. wassomewhat allied to C.( O.) okinawensis Arnaud, 1956, but was distinctly different from wing length 1.38 mm, antennasensilla coeloconica present on segments 3-14, antennal ratio (AR)1.28, mandible with 17 teeth of female; and shape of aedeagus, parameres, and basistyle with ventral root of male of C. ( O.) okinawensis Arnaud, 1956. The type specimens are deposited in the PLA Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

Two new species of the Culicoides(Oecacta), C.(O.) mengyuanensis sp. nov.,and C.(O.) sublini sp. nov. are described from Yunnan and Tibet in China. C.(O.) mengyuanensis sp. nov. is somewhat allied to C.(O.) yadongensis Chu, 1977 in the eyes and buccal armature, but is distinctly different from antenna sensilla coeloconica present on segments 3, 11-15, antennal ratio(AR)1.04 of female C.(O.) yadongensis Chu, 1977. C.(O.) sublini sp. nov. is similar to C.(O.) lini Kitaoka et Tanaka, 1985, both are distinctly different in the small-sized species(wing length 0.93 mm), eyes with interfacetal hairs, wing base portion with a pale spot, and contiguity with a pale spot in basal portion of Anal cell; wing with a pale spot over basal potion half of R1 cell and r-m cross-vein, and separated with a pale spot in middle potion of M2 cell of female C. lini Kitaoka et Tanaka, 1985. The type specimens are deposited in the Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

Objective To study species distribution, community composition and bloodsucking rate of hematophagous midges in 3 cities (countries) of China-Laos border. Methods Hematophagous midges were captured with light trap to generate data on the species distribution in China-Laos border in June to August, 2015. Results A total of 78 336 host-seeking midges belonging to 54 species and 3 genera were collected; genus Leptoconops with 4 species, genus Lasiohelea with 7 species, genus Culicoedes with 43 species. Seven species, C. gentiloides, C. huffi, C. kibabaluensis, C. lansangensis, C. parahumeralis, C. variatus, La. adita are recorded for the first time in Yunnan province. The species distribution of hematophagous midges was recorded 48 species in Mengla, 22 species in Jiangcheng, 16 species in Jinghong. The predominant species included C. jacobsoni, C. parahumeralis and C. palpifer, and their composition were 25.93%, 19.25%, and 17.13% respectively. The community composition and the bloodsucking rate of midges varied with different counties and habitats. The bloodsucking rate was 32.26% in residence district, and 28.24% in cowshed. Conclusion The investigation provides the reference for species distribution, community composition and studies of infectious disease by hematophagous Culicoides.

This paper reports 3 genera and 71 species of hematophagous midges in 17 cities and counties of bordering area of North Korea, Russia and China, among which a new species Culicoides moheensis Ren et Liu sp. nov. was described. Three genera and 42 species were distributed in the 7 cities and counties of China-North Korea borders; and 2 genera 54 species in 10 cities and counties of China-Russia borders. Culicoides raoheensis Ren et Liu, sp. nov. is similar to C. qianshanensis Fei, 1982, but distinctly different in the palpus third segment with a large and deep sensory pit, antenna sensilla coeloconica present on segments 3-15 of female C. qinashanensis Fei, 1982. The type specimen is deposited at the Shenyang Command Center for Disease Control and Prevention(Shenyang 110034, China).

The paper reports 1 genera and 19 species of bloodsucking midges in Hongxing volcano lava area, Heilongjiang province. A new species Culioides hongxingensis Yang et Liu sp. nov. is described. This new species is closely allied to C. pallidulus Yu, 1982, but is distinctly different from the eyes with interfacetal hairs, eyes are separated by width of 3.5 facet of female C. pallidulus Yu, 1982. The new species is also similar to C. subcircumscriptus Yu, 1991, but is distinctly different from the middle of pale spot over x-vein with small dark spot, cell M1 and cell M2 without pale spot of C. subcircumscriptus Yu, 1991. Culicoides donggangensis Liu, Zhou et Wu, 2007 is recorded for the first time in Heilongjiang province. The type specimens are deposited in the Shenyang Command Center for Disease Control and Prevention (Shenyang 110034, China).

Objective To investigate the important medical arthropods from Haerbaling and Lianhuapao forest areas of Dunhua city, Jilin province. Methods Blood-sucking Diptera insects were collected by bednet, light trap and insect net. Ticks were collected by dragging a blanket over the vegetation on the ground. Results A total of 16 141 specimens belonging 4 families 12 genera 50 species were recorded from Dunhua city, including 10 species and 3 genera of Culicidae, 8 species and 1 genus of Ceratopogonidae (including a new species: Culicoides haerbalingensis), 28 species and 5 genera of Tabanidae, 4 species and 3 genera of Ixodidae. The predominant species were Aedes vexans (81.96％); Culicoides newsteadi (48.01％), Culicoides punctatus (24.20％); Hybomitra distinguenda (26.17％), Hybomitra stenopselaphas (12.66％); Haema physalis concinna (67.34％). The peak period of ticks was May. Conclusion There were many medical arthropods and hazard in forest areas of Dunhua city, and medical arthropods should be controlled.

Objective To study the species distribution and taxonomy of Culicoides (Fastus) in China. Methods Midges were captured using bed nets, light traps, and insect nets. Results A total of 12 species of Culicoides (Fastus) were recorded in China, including Culicoides (Fastus) mingshanensis sp. nov. The distribution of the 12 known species in China and the measurements of their major differential characteristics were described to compile an index list. Conclusion This study provides a reference for investigating the species distribution and taxonomy of Culicoides (Fastus) in China. C. mingshanensis sp. nov. has similar wing spots as C. festivipennis Kieffer, 1914, but the latter is distinctly different in the sensilla coeloconica of antenna situated on segments 3-15 and the third segment of palpus with sensory pit. The type specimen of the new species is deposited at the Shenyang Military Command Centre for Disease Control and Prevention (Shenyang 110034, China).

Objective To determine the species of hematophagous midges in Luobei county, Heilongjiang province, China.Methods Light traps and insect nets were used to capture the midges. Results A total of 26 827 bloodsucking midges (30 species, one genus) were captured in Luobei county from 2009 to 2013. A new species of the genus Culicoides, C. luobeiensis Liang et Liu, sp. nov., was reported. The new species was closely allied to C. subcircumscriptus Yu, 1982, but C. subcircumscriptus Yu, 1982 had the following distinct differences: presence of little pale spots on cell R5 and the distal portion of vena M1; in females, presence of sensilla coeloconica on segments 3, 11-14; in males, presence of spiculae on the ventral membrane of the ninth sternum, stout apicolateral processes on the ninth tergum, absence of the ventral root of basistyle, and absence of small pricks inside the paramere bases. C. alazanicus Dzhafarov, 1961 (♀) was recorded for the first time in China. Conclusion This investigation provides a basis for further study on the species distribution of hematophagous midges in Luobei county, Heilongjiang province. The type specimens are deposited in the Shenyang Military Command Center for Disease Control and Prevention (Shenyang 110034, China).

Objective To determine the species of hematophagous midges in Luobei county, Heilongjiang province, China. Methods Light traps and insect nets were used to capture the midges. Results A total of 26 827 bloodsucking midges (30 species, one genus) were captured in Luobei county from 2009 to 2013. A new species of the genus Culicoides, C. luobeiensis Liang et Liu, sp. nov., was reported. The new species was closely allied to C. subcircumscriptus Yu, 1982, but C. subcircumscriptus Yu, 1982 had the following distinct differences: presence of little pale spots on cell R5 and the distal portion of vena M1; in females, presence of sensilla coeloconica on segments 3, 11-14; in males, presence of spiculae on the ventral membrane of the ninth sternum, stout apicolateral processes on the ninth tergum, absence of the ventral root of basistyle, and absence of small pricks inside the paramere bases. C. alazanicus Dzhafarov, 1961 (♀) was recorded for the first time in China. Conclusion This investigation provides a basis for further study on the species distribution of hematophagous midges in Luobei county, Heilongjiang province. The type specimens are deposited in the Shenyang Military Command Center for Disease Control and Prevention (Shenyang 110034, China).

Objective To investigate the species of bloodsucking midges in Xunke county, Heilongjiang province, China. Methods Light traps and insect nets were used to capture bloodsucking midges. Results A total 7810 bloodsucking midges (16 species, one genus) were captured in Xunke county, and female Culicoides xunkeensis Yang, Li et Liu, 2011 was newly found. Conclusion This investigation provides a basis for the study on species of bloodsucking midges in Xunke county, Heilongjiang province. The specimens are deposited in the Xunke Entry-Exit Inspection and Quarantine Bureau.

Objective To investigate the species composition of mosquitoes and the diurnal variations in numbers of dominant species of mosquitoes on the Heixiazi Island in Fuyuan county, Heilongjiang province, China. Methods Mosquitoes were collected using the human-baited net trap, light trap, and insect net. Results A total of 33 526 mosquitoes belonging to 4 genera and 10 species were collected on the Heixiazi Island. The dominant species was Aedes vexans (94.68%). The species composition varied in different months. The diurnal variations in numbers of 4 dominant species of mosquitoes were as follows: Ae. vexans was active in a whole day, Ae. sticticus during the 3rd to 21st hour of day, Culex modestus during the 6th to 20th hour, and Anopheles sinensis during the 3rd to 19th hour; the activity peak occurred at the 3rd and 20th hours for Ae. vexans, at the 17th hour for Cx. modestus, at the 18th hour for Ae. sticticus, and at the 21st hour for An. sinensis. Conclusion The mosquitoes on the Heixiazi Island were composed of 4 genera and 10 species, and the dominant species was Aedes vexans, and the diurnal-variation-number of the four dominant species was observed.

A new species of Culicoides ( Oecacta) was detected in the identification of the specimens of bloodsucking midges from Shandong province, C. ( O.) shandongensis sp. nov. The new species was somewhat allied to C. ( O.) zhuhaiensis Yu et Hao, 1988, but distinctly different in the shape of the third segment of the palpus, the pale sport of the distal portion of cell R2, and the female spermathecae. C. ( O.) turanicus Gutsevich et Smatov,1971 was recorded for the first time in China. The type specimen is deposited in the Center for Disease Control and Prevention of Shenyang PLA Command, Shenyang 110034, China.

A new species of the genus Culicoides, C. liweiae sp. nov. from Luobei port, Heilongjiang province, China is reported. The new species is closely allied to male C. gornostaevae Mirzaeva, 1984, but what is distinctly different between them is that the latter is pale in the distal half of cell R2 with a nearly triangle pale spot in the distal portion of cell R wing, no mocrotrichia in the basal cell of wing, the ventra root of two lateral basistly not continuous, and the ninth sternum in“V”caudomedion excavation. The ninth sternum of the male of this new species is also similar to that of C. sinanoensis Tokunaga, 1937, but they are distinctly different in the shapes of ventra root basistly, ninth tergum, and aedeagus. C. filicinus Gornostaeva et Gachegova, 1972 (♀) is recorded for the first time in China. The type specimen is deposited in the Entomology Collection Gallection Gallery of Heilongjiang Entry-Exit Inspection and Quarantine Brueau of the P. R. China (Haerbin 150001, China).

Objective To investigate the species distribution and population composition of bloodsucking mosquitoes and midges at Raohe port in Heilongjiang province. Methods Bed curtains, light traps and insect nets were used to collect mosquitoes and bloodsucking midges. Results From 2008 to 2009, 90 214 mosquitoes and bloodsucking midges were captured in the region, which belonged to 63 species of 6 genera, 2 families, including 28 species, 4 genera of Culicoidae and 35 species, 2 genera of Ceratopogonidae. Culicoides haerbalingensis were recorded for the first time at Raohe port. The predominant species of mosquitoes was Aedes vaxans (84.94%). The predominant species of bloodsucking midges were Culicoides sinanoennis (37.19%), C. obsoletus (20.03%) and C. punctatus (20.04%). The population composition of mosquitoes and bloodsucking midges varied in different habitats. Conclusion This investigation provided baseline data on bloodsucking mosquitoes and midges for formulation of the prevention and control strategies.